产品货号：
Z37175
中文名称：
KAP1/TRIM28抗体
英文名称：
Anti-KAP1/TRIM28 Antibody (Clone#3H2)
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-KAP1/TRIM28 Antibody (Clone#3H2) |
| 指标别名 | KAP 1;KAP1;KRAB associated protein 1;KRAB interacting protein 1;KRIP 1;Nuclear corepressor KAP 1;RING finger protein 96;RNF96;TF1B;TIF1 beta;TIF1B;TRIM28;tripartite motif containing 28 |
| 克隆性 | Monoclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,IHC-F,IF,ICC/IF,FCM |
| 基因名称 | TRIM28 |
| 克隆号 | 3H2 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG2b |
| 免疫原 | E.coli-derived human KAP1 recombinant protein (Position:A699-P835).Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1. |
| 实际分子量 | 100KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Tripartite motif-containing 28 (TRIM28),also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1),is a protein that in humans is encoded by the TRIM28 gene.The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors.The protein localizes to the nucleus and is thought to associate with specific chromatin regions.KAP1 is a ubiquitously expressed protein involved in many critical functions including:transcriptional regulation,cellular differentiation and proliferation,DNA damage repair,viral suppression,and apoptosis.Its functionality is dependent upon post-translational modifications.Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator. |
| Uniprot ID | Q13263 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (ZN1928) for Western blot,and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (ZN1866) for IHC(P) and ICC. |
| 基因名全称 | tripartite motif containing 28 |
| 蛋白名全称 | Transcription intermediary factor 1-beta |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry(Paraffin-embedded Section) 1:50～400 Immunohistochemistry(Frozen Section) 1:50～400 Immunocytochemistry/Immunofluorescence(ICC/IF) 1:50～400 Immunofluorescence (IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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